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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SBDS Monoclonal antibody (67200-1-Ig)

    WB analysis using 67200-1-Ig (same clone as 67200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67200-1-Ig (SBDS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SBDS Monoclonal antibody (67200-1-Ig)

    IHC staining of human breast cancer using 67200-1-Ig (same clone as 67200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67200-1-Ig (SBDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SBDS Monoclonal antibody (67200-1-Ig)

    IHC staining of human breast cancer using 67200-1-Ig (same clone as 67200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67200-1-Ig (SBDS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SBDS Monoclonal antibody (67200-1-Ig)

    IF Staining of HepG2 using 67200-1-Ig (same clone as 67200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SBDS antibody (67200-1-Ig, Clone: 1F8A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SBDS Monoclonal antibody (67200-1-Ig)

    IF Staining of HepG2 using 67200-1-Ig (same clone as 67200-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SBDS antibody (67200-1-Ig, Clone: 1F8A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SBDS Monoclonal antibody, PBS Only

SBDS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67200-1-PBS
Clone No.1F8A10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1F8A10, CGI 97, CGI-97, SDS, Shwachman-Bodian-Diamond syndrome protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67200-1-PBS targets SBDS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11731

Product name: Recombinant human SBDS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-250 aa of BC065700

Sequence: MSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIESEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE

相同性解析による交差性が予測される生物種
Full Name Shwachman-Bodian-Diamond syndrome
Calculated molecular weight 250 aa, 29 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC065700
Gene Symbol SBDS
Gene ID (NCBI) 51119
RRIDAB_2882493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3A5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined.

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