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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAV1 Polyclonal antibody (32277-1-AP)

    WB analysis using 32277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32277-1-AP (SAV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SAV1 Polyclonal antibody (32277-1-AP)

    IHC staining of rat kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SAV1 Polyclonal antibody (32277-1-AP)

    IHC staining of mouse kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SAV1 Polyclonal antibody (32277-1-AP)

    IHC staining of mouse kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SAV1 Polyclonal antibody (32277-1-AP)

    IHC staining of rat kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SAV1 Polyclonal antibody (32277-1-AP)

    IF Staining of A549 using 32277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SAV1 antibody (32277-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SAV1 Polyclonal antibody

SAV1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32277-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, HUVEC cells, U2OS cells
Positive IHC detected inmouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32277-1-AP targets SAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAV1 fusion protein Ag36278 相同性解析による交差性が予測される生物種
Full Name salvador homolog 1 (Drosophila)
Calculated molecular weight 383 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC020537
Gene Symbol SAV1
Gene ID (NCBI) 60485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H4B6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343).

Protocols

Product Specific Protocols
WB protocol for SAV1 antibody 32277-1-APDownload protocol
IHC protocol for SAV1 antibody 32277-1-APDownload protocol
IF protocol for SAV1 antibody 32277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols