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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SATB2 Polyclonal antibody (30717-1-AP)

    WB analysis using 30717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30717-1-AP (SATB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SATB2 Polyclonal antibody (30717-1-AP)

    IHC staining of human colon cancer using 30717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30717-1-AP (SATB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SATB2 Polyclonal antibody

SATB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30717-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

DNA binding protein SATB2, DNA-binding protein SATB2, FLJ21474, FLJ32076, KIAA1034

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30717-1-AP targets SATB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SATB2 fusion protein Ag33523 相同性解析による交差性が予測される生物種
Full Name SATB homeobox 2
Calculated molecular weight 733 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC098136
Gene Symbol SATB2
Gene ID (NCBI) 23314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

Protocols

Product Specific Protocols
WB protocol for SATB2 antibody 30717-1-APDownload protocol
IHC protocol for SATB2 antibody 30717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols