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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb™ SAT1 Multi-Recombinant antibody (RMX00008)

    WB analysis using RMX00008

    Untreated and DENSPM treated HEK-293 cells were were subjected to SDS PAGE followed by western blot with RMX00008 (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Multi-rAb™ SAT1 Multi-Recombinant antibody (RMX00008)

    IHC staining of mouse brain using RMX00008

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00008 (SAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Multi-rAb™ SAT1 Multi-Recombinant antibody (RMX00008)

    IF Staining of RAW 264.7 using RMX00008

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using SAT1 antibody (RMX00008) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Multi-rAb™ SAT1 Multi-Recombinant antibody

SAT1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00008

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SSAT 1, SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00008 targets SAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name spermidine/spermine N1-acetyltransferase 1
Calculated molecular weight 20 kDa
Observed molecular weight 15-25 kDa
GenBank accession numberBC008424
Gene Symbol SAT1
Gene ID (NCBI) 6303
Conjugate Unconjugated
Form Liquid
Purification MethodN/A
UNIPROT IDP21673
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SAT1 antibody RMX00008Download protocol
IHC protocol for SAT1 antibody RMX00008Download protocol
IF protocol for SAT1 antibody RMX00008Download protocol
Standard Protocols
Click here to view our Standard Protocols