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- KD/KO Validated
SAT1 Polyclonal antibody
SAT1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10708-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, mouse liver tissue, HEK-293 cell, HeLa cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 16 publications below |
| IHC | See 6 publications below |
| IF | See 1 publications below |
Product Information
10708-1-AP targets SAT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SAT1 fusion protein Ag1154 相同性解析による交差性が予測される生物種 |
| Full Name | spermidine/spermine N1-acetyltransferase 1 |
| Calculated molecular weight | 20 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC008424 |
| Gene symbol | SAT1 |
| Gene ID (NCBI) | 6303 |
| RRID | AB_2877739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SAT1 antibody 10708-1-AP | Download protocol |
| IHC protocol for SAT1 antibody 10708-1-AP | Download protocol |
| IP protocol for SAT1 antibody 10708-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Urea cycle activation triggered by host-microbiota maladaptation driving colorectal tumorigenesis | ||
Proc Natl Acad Sci U S A Polyamine pathway contributes to the pathogenesis of Parkinson disease. | ||
Mol Neurobiol Electroacupuncture Alleviates Neuropathic Pain by Suppressing Ferroptosis in Dorsal Root Ganglion via SAT1/ALOX15 Signaling | ||
Food Chem Toxicol DEHP induces ferroptosis in testes via p38α-lipid ROS circulation and destroys the BTB integrity. | ||
Biochim Biophys Acta Mol Cell Res Urea transporter B downregulates polyamines levels in melanoma B16 cells via p53 activation. | ||
PLoS One Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles. |
