Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 21377-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 21377-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21377-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21377-1-AP
IP result of anti-SASS6 (IP:21377-1-AP, 4ug; Detection:21377-1-AP 1:1000) with HeLa cells lysate 600ug.
IP experiment of HeLa cells using 21377-1-AP
IP result of anti-SAS6 (21377-1-AP for IP and Detection) with HeLa cells.
IHC staining of human kidney using 21377-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 21377-1-AP (SASS6 Antibody) at dilution of 1:50.
IHC staining of mouse testis using 21377-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 21377-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SASS6 Polyclonal antibody

SASS6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21377-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HsSAS 6, HsSAS-6, SAS 6, SAS6, Spindle assembly abnormal protein 6 homolog

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human testis tissue, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

21377-1-AP targets SASS6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SASS6 fusion protein Ag15917 相同性解析による交差性が予測される生物種
Full Name	spindle assembly 6 homolog (C. elegans)
Calculated molecular weight	657 aa, 74 kDa
Observed molecular weight	70-74 kDa
GenBank accession number	BC101026
Gene Symbol	SASS6
Gene ID (NCBI)	163786
RRID	AB_2878851
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6UVJ0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Spindle assembly abnormal protein 6 homolog (SASS6) is a centrosomal protein that, together with PLK4, CEP135, CENPJ (also known as CPAP) and STIL, is involved in regulating the number of centrosomes in human cells.

Protocols

Product Specific Protocols
WB protocol for SASS6 antibody 21377-1-AP	Download protocol
IHC protocol for SASS6 antibody 21377-1-AP	Download protocol
IP protocol for SASS6 antibody 21377-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. Authors - Hindol Gupta View Article
human	WB,IHC	Oncol Rep Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase. Authors - Lili Du View Article
human	IF	Science The mechanism of acentrosomal spindle assembly in human oocytes Authors - Tianyu Wu View Article
human	IF	EBioMedicine Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities Authors - Niu Li View Article
human	WB	Clin Exp Med SASS6 promotes tumor proliferation and is associated with TP53 and immune infiltration in lung adenocarcinoma Authors - Zihao Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 21377-1-AP

WB analysis of human testis using 21377-1-AP

WB analysis of MCF-7 using 21377-1-AP

IP experiment of HeLa using 21377-1-AP

IP experiment of HeLa cells using 21377-1-AP

IHC staining of human kidney using 21377-1-AP

IHC staining of mouse testis using 21377-1-AP

IHC staining of mouse testis using 21377-1-AP

SASS6 Polyclonal antibody

SASS6 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.

Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.

The mechanism of acentrosomal spindle assembly in human oocytes

Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities

SASS6 promotes tumor proliferation and is associated with TP53 and immune infiltration in lung adenocarcinoma