Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31915-1-AP targets SASH3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36154 Product name: Recombinant human SASH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-380 aa of BC051881 Sequence: DVLPEEAVGHARPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELRETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESGRDDAELAGTEEQLQGLSLAGAP 相同性解析による交差性が予測される生物種 |
| Full Name | SAM and SH3 domain containing 3 |
| Calculated molecular weight | 380 aa, 42 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC051881 |
| Gene Symbol | SASH3 |
| Gene ID (NCBI) | 54440 |
| RRID | AB_3742493 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O75995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sterile alpha motif (SAM) and Src homology-3 (SH3) domain-containing 3 (SASH3), also called SH3-containing lymphocyte protein (SLY1), is a putative adaptor protein that is postulated to play an important role in the organization of signaling complexes and propagation of signal transduction cascades in lymphocytes. The SASH3 gene is located on the X-chromosome, which plays an important role in human lymphocyte function and survival (PMID: 33876203).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SASH3 antibody 31915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |