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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SARM1 Polyclonal antibody (28625-1-AP)

    WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SARM1 Polyclonal antibody (28625-1-AP)

    WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SARM1 Polyclonal antibody (28625-1-AP)

    WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SARM1 Polyclonal antibody (28625-1-AP)

    IHC staining of rat brain using 28625-1-AP (same clone as 28625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28625-1-AP (SARM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SARM1 Polyclonal antibody (28625-1-AP)

    IHC staining of rat brain using 28625-1-AP (same clone as 28625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28625-1-AP (SARM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SARM1 Polyclonal antibody (28625-1-AP)

    IF Staining of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody (28625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using SARM1 Polyclonal antibody (28625-1-AP)

    FC experiment of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody (28625-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

SARM1 Polyclonal antibody, PBS Only

SARM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 28625-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:3.2.2.-, EC:3.2.2.6, FLJ36296, hSARM1, HsTIR

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28625-1-PBS targets SARM1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29531

Product name: Recombinant human SARM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 352-573 aa of NM_015077

Sequence: EAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKEAEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPCTGGKPSGDTPDVFISYRRNSG

相同性解析による交差性が予測される生物種
Full Name sterile alpha and TIR motif containing 1
Calculated molecular weight 79 kDa
Observed molecular weight70-80 kDa
GenBank accession numberNM_015077
Gene Symbol SARM1
Gene ID (NCBI) 23098
RRIDAB_3086070
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6SZW1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing 1) is also named as KIAA0524, SAMD2 and SARM. SARM1 is the major pro-degenerative protein in axons (PMID: 32152523). SARM1 is the central executioner of pathological axon degeneration and is an inducible NAD+-cleavage enzyme that is activated by the loss of the NAD+ biosynthetic enzyme NMNAT2 (PMID: 33657413). SARM1 is involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PMID: 16964262).

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