Validation Data Gallery
Tested Applications
| Positive WB detected in | human peripheral blood platelets, mouse lung tissue, rat lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16056-1-AP targets SAP30L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9024 Product name: Recombinant human SAP30L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC009829 Sequence: MNGFSTEEDSREGPPAAPAAAAPGYGQSCCLIEDGERCVRPAGNASFSKRVQKSISQKKLKLDIDKSVRHLYICDFHKNFIQSVRNKRKRKTSDDGGDSPEHDTDIPEVDLFQLQVNTLRRYKRHYKLQTRPGFNKAQLAETVSRHFRNIPVNEKETLAYFIYMVKSNKSRLDQKSEGGKQLE 相同性解析による交差性が予測される生物種 |
| Full Name | SAP30-like |
| Calculated molecular weight | 183 aa, 21 kDa |
| Observed molecular weight | 21-25 kDa |
| GenBank accession number | BC009829 |
| Gene Symbol | SAP30L |
| Gene ID (NCBI) | 79685 |
| RRID | AB_3085479 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAJ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SAP30L, also named as NS4ATP2, is one of the key proteins in a multi‐subunit protein complex involved in transcriptional regulation via histone deacetylation. SAP30L is an essential component of the Sin3A corepressor complex. The MW migrates to 26-30 kDa with phospho and SUMO modification and may generate complexes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SAP30L antibody 16056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |