• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HEK-293 using 12130-1-AP (same clone as 12130-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HeLa using 12130-1-AP (same clone as 12130-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of Jurkat using 12130-1-AP (same clone as 12130-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of K-562 using 12130-1-AP (same clone as 12130-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SAP130 Polyclonal antibody (12130-1-AP)

    IP experiment of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    IP result of anti-SAP130 (IP:12130-1-AP, 4ug; Detection:12130-1-AP 1:600) with HepG2 cells lysate 1000ug. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SAP130 Polyclonal antibody (12130-1-AP)

    IHC staining of human pancreas cancer using 12130-1-AP (same clone as 12130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12130-1-AP (SAP130 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAP130 Polyclonal antibody (12130-1-AP)

    IF Staining of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAP130 antibody (12130-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

SAP130 Polyclonal antibody, PBS Only

SAP130 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12130-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Sin3-associated polypeptide p130, 130 kDa Sin3-associated polypeptide

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12130-1-PBS targets SAP130 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2774

Product name: Recombinant human SAP130 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 354-693 aa of BC017453

Sequence: PTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQHVISTEEGDMMETNSTDDEKSTAKSLLVKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLLRHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTNLEHDVYERLTNLQEGIIPKKKAATDDDLHRINELIQGNMQRCKLVMDQISEARDSMLKVLDHKDRVLKLLNKNGTVKKVSKLKRKEKV

相同性解析による交差性が予測される生物種
Full Name Sin3A-associated protein, 130kDa
Calculated molecular weight 110 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC017453
Gene Symbol SAP130
Gene ID (NCBI) 79595
RRIDAB_2186637
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0E3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

||
New chat

Able™

正在加载，请稍候...