Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
55085-1-AP targets SAP 97 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | discs, large homolog 1 (Drosophila) |
| Calculated molecular weight | 100 kDa |
| Observed molecular weight | 120 kDa, 130-140 kDa |
| GenBank accession number | NM_004087 |
| Gene Symbol | SAP97 |
| Gene ID (NCBI) | 1739 |
| RRID | AB_11182375 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12959 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.
Publications
| Species | Application | Title |
|---|---|---|
Kidney Int Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney. | ||
Neurochem Res PFOS Elicits Cytotoxicity in Neuron Through Astrocyte-Derived CaMKII-DLG1 Signaling In Vitro Rat Hippocampal Model |