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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAP 97 Polyclonal antibody (29292-1-AP)

    WB analysis using 29292-1-AP (same clone as 29292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29292-1-AP (DLG1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SAP 97 Polyclonal antibody (29292-1-AP)

    WB analysis of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

    WB result of SAP 97 antibody (29292-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAP 97 transfected HEK-293 cells. This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SAP 97 Polyclonal antibody (29292-1-AP)

    IHC staining of human colon cancer using 29292-1-AP (same clone as 29292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SAP 97 Polyclonal antibody (29292-1-AP)

    IHC staining of human colon cancer using 29292-1-AP (same clone as 29292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SAP 97 Polyclonal antibody (29292-1-AP)

    IHC staining of human breast cancer using 29292-1-AP (same clone as 29292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SAP 97 Polyclonal antibody (29292-1-AP)

    IF Staining of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SAP 97 antibody (29292-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SAP 97 Polyclonal antibody, PBS Only

SAP 97 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29292-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DLG1, Disks large homolog 1, SAP-97, SAP97, hdlg

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29292-1-PBS targets SAP 97 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31066

Product name: Recombinant human DLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of NM_001098424

Sequence: MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVINIFQSNLFQALIDIQEFYEVTLLDNPKCIDRSKPSEPIQPVNTWEISSLPSSTVTSETLPSSLSPSVEKYRYQDEDTPPQEHISPQITNEVIGPELVHVSEKNLSEIENVHGFVSHSHISPIKPTEAVLPSPPTVPVIPVLPVPAENTVILPTIPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLER

相同性解析による交差性が予測される生物種
Full Name discs, large homolog 1 (Drosophila)
Calculated molecular weight 100 kDa
Observed molecular weight100-130 kDa
GenBank accession numberNM_001098424
Gene Symbol SAP97
Gene ID (NCBI) 1739
RRIDAB_2918273
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12959
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.

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