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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SAMSN1 Polyclonal antibody (13063-1-AP)

    WB analysis of K-562 using 13063-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13063-1-AP (SAMSN1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SAMSN1 Polyclonal antibody (13063-1-AP)

    WB analysis of K-562 using 13063-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13063-1-AP (SAMSN1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using SAMSN1 Polyclonal antibody (13063-1-AP)

    IP experiment of K-562 using 13063-1-AP

    IP result of anti-SAMSN1 (IP:13063-1-AP, 4ug; Detection:13063-1-AP 1:1000) with K-562 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SAMSN1 Polyclonal antibody (13063-1-AP)

    IHC staining of human gliomas using 13063-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13063-1-AP (SAMSN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SAMSN1 Polyclonal antibody (13063-1-AP)

    IHC staining of human gliomas using 13063-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13063-1-AP (SAMSN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAMSN1 Polyclonal antibody (13063-1-AP)

    IF Staining of HepG2 using 13063-1-AP

    Immunofluorescent analysis of HepG2 cells using 13063-1-AP (SAMSN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

SAMSN1 Polyclonal antibody

SAMSN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13063-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SH3-SAM adaptor protein, SH3D6B, SH3 SAM adaptor protein, SAM domain-containing protein SAMSN-1, HACS1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13063-1-AP targets SAMSN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3755

Product name: Recombinant human SAMSN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC029112

Sequence: MLKRKPSNVSEKEKHQKPKRSSSFGNFDRFRNNSLSKPDDSTEAHEGDPTNGSGEQSKTSNNGGGLGKKMRAISWTMKKKVGKKYIKALSEEKDEEDGENAHPYRNSDPVIGTHTEKVSLKASDSMDSLYSGQSSSSGITSCSDGTSNRDSFRLDDDGPYSGPFCGRARVHTDFTPSPYDTDSLKIKKGDIIDIICKTPMGMWTGMLNNKVGNFKFIYVDVISEEEAAPKKIKANRRSNSKKSKTLQEFLERIHLQEYTSTLLLNGYETLEDLKDIKESHLIELNIENPDDRRRLLSAAENFLEEEIIQEQENEPEPLSLSSDISLNKSQLDDCPRDSGCYISSGNSDNGKEDLESENLSDMVHKIIITEPSD

相同性解析による交差性が予測される生物種
Full Name SAM domain, SH3 domain and nuclear localization signals 1
Calculated molecular weight 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC029112
Gene Symbol SAMSN1
Gene ID (NCBI) 64092
RRIDAB_2877910
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSI8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SAMSN1 antibody 13063-1-APDownload protocol
IHC protocol for SAMSN1 antibody 13063-1-APDownload protocol
IP protocol for SAMSN1 antibody 13063-1-APDownload protocol
WB protocol for SAMSN1 antibody 13063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation

Authors - Zoe Schaefer
View Article
humanIHC

Ann Surg Oncol

Suppression of SAMSN1 Expression is Associated with the Malignant Phenotype of Hepatocellular Carcinoma.

Authors - Satoshi Sueoka
View Article
mouseWB

Stem Cells Int

Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation

Authors - Lei Zhang
  • KD Validated
View Article
humanIHC

Oncol Lett

Prognostic relevance of SAMSN1 expression in gastric cancer.

Authors - Mitsuro Kanda
View Article
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