Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 20824-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 20824-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20824-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 20824-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 20824-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 20824-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human hepatocirrhosis using 20824-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 20824-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 20824-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 20824-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20824-1-AP (Sam50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20824-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, A375 cells, mouse ovary tissue, mouse kidney tissue
Positive IHC detected in	human hepatocirrhosis tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IF	See 1 publications below

Product Information

20824-1-AP targets Sam50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14799 Product name: Recombinant human SAMM50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-468 aa of BC007830 Sequence: TFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSMHSIGPQSEGDYLGGEAYWAGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL 相同性解析による交差性が予測される生物種
Full Name	sorting and assembly machinery component 50 homolog (S. cerevisiae)
Calculated molecular weight	469 aa, 52 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC007830
Gene Symbol	Sam50
Gene ID (NCBI)	25813
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y512
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Protocols

Product Specific Protocols
IF protocol for Sam50 antibody 20824-1-AP	Download protocol
IHC protocol for Sam50 antibody 20824-1-AP	Download protocol
WB protocol for Sam50 antibody 20824-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Elife QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. Authors - Virginia Guarani View Article
human	WB	Cell Rep A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells. Authors - Timothy D Martin KD Validated View Article
mouse	WB	Aging Cell 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex Authors - Zer Vue View Article
rat	WB	Free Radic Biol Med Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy. Authors - Run-Qing Xue View Article
human	WB	J Cell Biol The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy Authors - Mehmet Oguz Gok View Article
pig	IF	Elife Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system Authors - Dalen Zuidema View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20824-1-AP

WB analysis of A375 using 20824-1-AP

WB analysis of HEK-293 using 20824-1-AP

WB analysis of mouse ovary using 20824-1-AP

WB analysis of A431 using 20824-1-AP

IHC staining of human hepatocirrhosis using 20824-1-AP

IHC staining of human hepatocirrhosis using 20824-1-AP

IHC staining of human skin using 20824-1-AP

IHC staining of human skin using 20824-1-AP

IF Staining of HeLa using 20824-1-AP

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex

Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.

The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system