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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of HeLa using 17723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of mouse brain using 17723-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of mouse liver using 17723-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of mouse kidney using 17723-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of human urothelial carcinoma using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of human urothelial carcinoma using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of human thyroid cancer using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of human thyroid cancer using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of mouse kidney using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of mouse pancreas using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of rat kidney using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of rat pancreas using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    IHC staining of rat small intestine using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAMD4B Polyclonal antibody (17723-1-AP)

    IF Staining of HepG2 using 17723-1-AP

    Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Featured Product
  • KD/KO Validated

SAMD4B Polyclonal antibody

SAMD4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17723-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

hSmaug2, Protein Smaug homolog 2, SAM domain-containing protein 4B, Smaug 2, SMAUG2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17723-1-AP targets SAMD4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12113

Product name: Recombinant human SAMD4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 343-694 aa of BC054518

Sequence: KGARHKIALSIQKLRERQSVLKSLEKDVLEGGNLRNALQELQQIIITPIKAYSVLQATVAAATTTPTAKDGAPGEPPLPGAEPPLAHPGTDKGTEAKDPPAVENYPPPPAPAPTDGSEPAPAPVADGDIPSQFTRVMGKVCTQLLVSRPDEENITSYLQLIEKCLTHEAFTETQKKRLLSWKQQVLKLLRTFPRKAALEMQNYRQQKGWAFGSNSLPIAGSVGMGVARRTQRQFPMPPRALPPGRMGLLSPSGIGGVSPRHALTSPSLGGQGRQNLWFANPGGSNSMPSQSRSSVQRTHSLPVHSSPQAILMFPPDCPVPGPDLEINPTLESLCLSMTEHALGDGTDKTSTI

相同性解析による交差性が予測される生物種
Full Name sterile alpha motif domain containing 4B
Calculated molecular weight 694 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC054518
Gene Symbol SAMD4B
Gene ID (NCBI) 55095
RRIDAB_2183381
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5PRF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sterile alpha motif domain-containing protein 4B (SAMD4B) is also named as Smaug2. SAMD4B is a mammalian homologue of the Smaug protein of Drosophila melanogaster and can participate in the process of regulating RNA stability as a posttranscriptional inhibitor (PMID: 23592795). SAMD4B is expressed in most human embryos and adult tissue (PMID: 23592795). SAMD4B, especially its expression level, could affect the immune microenvironment (PMID: 38886351).

Protocols

Product Specific Protocols
WB protocol for SAMD4B antibody 17723-1-APDownload protocol
IHC protocol for SAMD4B antibody 17723-1-APDownload protocol
IF protocol for SAMD4B antibody 17723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Immunol

SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.

Authors - Yuze Wang
  • KO Validated
View Article
humanWB,IF

Mol Med Rep

miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B.

Authors - Chunrong Wu
View Article