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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IHC staining of human placenta using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IHC staining of human placenta using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IHC staining of rat testis using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IHC staining of rat testis using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IHC staining of mouse testis using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IHC staining of mouse testis using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IF Staining of mouse brain using 27761-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SALL1 antibody (27761-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SALL1 Polyclonal antibody (27761-1-AP)

    IF Staining of mouse brain using 27761-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SALL1 antibody (27761-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SALL1 Polyclonal antibody

SALL1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 27761-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

HSAL1, Sal 1, Sal like protein 1, SAL1, TBS

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse testis tissue, human placenta tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27761-1-AP targets SALL1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26766

Product name: Recombinant human SALL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 558-670 aa of NM_001127892

Sequence: MPLPPTLPSLIPFIKTEEPAPIPISHSATSPPGSVKSDSGGPESATRNLGGLPEEAEGSTLPPSGGKSEESGMVTNSVPTASSSVLSSPAADCGPAGSATTFTNPLLPLMSEQF

相同性解析による交差性が予測される生物種
Full Name sal-like 1 (Drosophila)
Calculated molecular weight 140 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_001127892
Gene Symbol SALL1
Gene ID (NCBI) 6299
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSC2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SALL1(Sal-like protein 1), also known as SAL1. It is located in the cytoplasm and nucleus. The protein is mainly expressed in the kidney. Transcriptional repressor involved in organogenesis. The protein plays an essential role in ureteric bud invasion during kidney development. The molecular weight of SALL1 is 140 kDa.

Protocols

Product Specific Protocols
IF protocol for SALL1 antibody 27761-1-APDownload protocol
IHC protocol for SALL1 antibody 27761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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