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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SAGE1 Polyclonal antibody (30914-1-AP)

    IHC staining of human skin cancer using 30914-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using SAGE1 Polyclonal antibody (30914-1-AP)

    IHC staining of rat testis using 30914-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SAGE1 Polyclonal antibody (30914-1-AP)

    IHC staining of mouse testis using 30914-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using SAGE1 Polyclonal antibody (30914-1-AP)

    IF Staining of A375 using 30914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SAGE1 Polyclonal antibody (30914-1-AP)

    IF Staining of U2OS using 30914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SAGE1 Polyclonal antibody

SAGE1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30914-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman skin cancer tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30914-1-AP targets SAGE1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34216

Product name: Recombinant human SAGE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 247-387 aa of NM_018666.2

Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

相同性解析による交差性が予測される生物種
Full Name sarcoma antigen 1
Calculated molecular weight99kd
GenBank accession numberNM_018666.2
Gene Symbol SAGE1
Gene ID (NCBI) 55511
RRIDAB_3669783
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NXZ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).

Protocols

Product Specific Protocols
IF protocol for SAGE1 antibody 30914-1-APDownload protocol
IHC protocol for SAGE1 antibody 30914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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