Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of MCF-7 using 21857-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 21857-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21857-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21857-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21857-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21857-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21857-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21857-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21857-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21857-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21857-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 21857-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.
IHC staining of mouse testis using 21857-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21857-1-AP (SAFB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21857-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.
IHC staining of mouse testis using 21857-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21857-1-AP (SAFB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 21857-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.
IHC staining of human testis using 21857-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.
IF Staining of A431 using 21857-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SAFB antibody (21857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SAFB Polyclonal antibody

SAFB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21857-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SAF B1, SAF B, HSP27 estrogen response element-TATA box-binding protein, HSP27 ERE-TATA-binding protein, HET

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells, A431 cells, HCT 116 cells, COLO 320 cells
Positive IHC detected in	mouse testis tissue, human testis tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 4 publications below

Product Information

21857-1-AP targets SAFB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16451 Product name: Recombinant human SAFB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 58-213 aa of BC126219 Sequence: MERLKKAIEDEGGNPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAEIDNGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEEPSLEPE 相同性解析による交差性が予測される生物種
Full Name	scaffold attachment factor B
Calculated molecular weight	917 aa, 103 kDa
Observed molecular weight	150 kDa
GenBank accession number	BC126219
Gene Symbol	SAFB
Gene ID (NCBI)	6294
RRID	AB_2878928
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15424
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport. hnRNPs also bind heterogeneous nuclear RNA (hnRNA), the transcripts produced by RNA polymerase II. SAFB (scaffold attachment factor B) is a nuclear matrix-associated protein that binds to matrix- or scaffold-associating regions (MARs or SARs) on DNA and interacts with RNA polymerase II and serine-/arginine-rich RNA processing factors (SR proteins). SAFB, also designated HAP (hnRNP A1 associated protein) and HET (HSP 27-ERE-TATA-binding protein) is a proven hnRNP protein that has a speckled distribution in the nucleus and, in response to stress agents such as heat shock, is recruited to a few, large nuclear granules, called perichromatin granules. SAFB also binds to the estrogen receptor (ER) and is expressed in several breast cancer cell lines at varying levels. Subsequently, SAFB may play a role in breast cancer by mediating cellular proliferation and division. This antibody specifically reacts whith the isoform 1 of human SAFB protein. SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SAFB is 103 kDa, but the modified SAFB is about 130-175 kDa.(PMID: 28469965, PMID: 26273616 )

Protocols

Product Specific Protocols
IF protocol for SAFB antibody 21857-1-AP	Download protocol
IHC protocol for SAFB antibody 21857-1-AP	Download protocol
WB protocol for SAFB antibody 21857-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Acta Neuropathol Commun RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases. Authors - Mahlon Collins View Article
human	IF	Neurobiol Dis ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity. Authors - Haiyan An View Article
mouse	IF	Mol Cell SAFB restricts contact domain boundaries associated with L1 chimeric transcription Authors - Yaqiang Hong View Article
human	IF	Cell Rep Stress-induced TDP-43 nuclear condensation causes splicing loss of function and STMN2 depletion Authors - Wan-Ping Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 21857-1-AP

WB analysis using 21857-1-AP

WB analysis of A431 using 21857-1-AP

WB analysis of HepG2 using 21857-1-AP

WB analysis of MCF-7 using 21857-1-AP

WB analysis of HEK-293 using 21857-1-AP

WB analysis of HeLa using 21857-1-AP

WB analysis of HeLa using 21857-1-AP

WB analysis of HeLa using 21857-1-AP

WB analysis of MCF-7 using 21857-1-AP

WB analysis of MCF-7 using 21857-1-AP

IHC staining of human breast cancer using 21857-1-AP

IHC staining of mouse testis using 21857-1-AP

IHC staining of human breast cancer using 21857-1-AP

IHC staining of mouse testis using 21857-1-AP

IHC staining of human testis using 21857-1-AP

IHC staining of human testis using 21857-1-AP

IF Staining of A431 using 21857-1-AP

SAFB Polyclonal antibody

SAFB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.

ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity.

SAFB restricts contact domain boundaries associated with L1 chimeric transcription

Stress-induced TDP-43 nuclear condensation causes splicing loss of function and STMN2 depletion