Validation Data Gallery
Tested Applications
| Positive WB detected in | serum from mouse injected with bacteria |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
13192-1-AP targets SAA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3869 Product name: Recombinant human SAA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-122 aa of BC020795 Sequence: MKLLTGLVFCSLVLSVSSRSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGHGAEDSLADQAANKWGRSGRDPNHFRPAGLPEKY 相同性解析による交差性が予測される生物種 |
| Full Name | serum amyloid A2 |
| Calculated molecular weight | 122 aa, 14 kDa |
| Observed molecular weight | 12-13 kDa |
| GenBank accession number | BC020795 |
| Gene Symbol | SAA2 |
| Gene ID (NCBI) | 6289 |
| RRID | AB_2877923 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DJI9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human SAA proteins are a group of apolipoproteins mainly found in the high density lipoprotein (HDL) portion of plasma. The serum amyloid A (SAA) protein is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under the regulation of inflammatory cytokines(PMID: 12011456). Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA2 protein(PMID: 1463770). This antibody can recognize SAA1 and SAA2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SAA antibody 13192-1-AP | Download protocol |
| IHC protocol for SAA antibody 13192-1-AP | Download protocol |
| WB protocol for SAA antibody 13192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Crohns Colitis Lysophosphatidylcholine-Induced Aberrant Adipogenesis in Mesenchymal Stem Cells and Impaired Antibacterial Function in Adipocytes of Creeping Fat | ||
J Allergy Clin Immunol Small Proline-Rich Protein 2A Drives Epithelial Remodeling in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via SAA2 Upregulation | ||
Inflammation Interleukin-1β Induces Intracellular Serum Amyloid A1 Expression in Human Coronary Artery Endothelial Cells and Promotes its Intercellular Exchange. | ||
Int Immunopharmacol Artesunate attenuates serum amyloid A-induced M1 macrophage differentiation through the promotion of PHGDH | ||
Oncol Lett Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma | ||
bioRxiv Single cell RNA sequencing reveals a shift in cell function and maturation of endogenous and infiltrating cell types in response to acute intervertebral disc injury |
