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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using S100A9 Recombinant antibody (83578-6-RR)

    IF Staining of RAW 264.7 using 83578-6-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using S100A9 antibody (83578-6-RR, Clone: 240539F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using S100A9 Recombinant antibody (83578-6-RR)

    FC experiment of A431 using 83578-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of Jurkat cells using S100A9 Recombinant antibody (83578-6-RR)

    FC experiment of Jurkat using 83578-6-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83578-6-RR

    Affinity and Kinetic Characterization of 83578-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-6-RR against Human S100A9 were performed. The affinity constant is 1.19 nM.

S100A9 Recombinant monoclonal antibody

S100A9 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83578-6-RR
Clone No.240539F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

240539F4, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inA431 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83578-6-RR targets S100A9 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25764

Product name: Recombinant human S100A9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-114 aa of BC047681

Sequence: KLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
GenBank accession numberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
RRIDAB_3671191
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
IF protocol for S100A9 antibody 83578-6-RRDownload protocol
FC protocol for S100A9 antibody 83578-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols