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Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using S100A3 Polyclonal antibody (12343-1-AP)

    WB analysis of A2780 using 12343-1-AP (same clone as 12343-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 12343-1-AP (S100A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using S100A3 Polyclonal antibody (12343-1-AP)

    WB analysis of HeLa using 12343-1-AP (same clone as 12343-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12343-1-AP (S100A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using S100A3 Polyclonal antibody (12343-1-AP)

    WB analysis of mouse skin using 12343-1-AP (same clone as 12343-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12343-1-AP (S100A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12343-1-PBS in a different storage buffer formulation.

S100A3 Polyclonal antibody, PBS Only

S100A3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12343-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12343-1-PBS targets S100A3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2995

Product name: Recombinant human S100A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-101 aa of BC012893

Sequence: MARPLEQAVAAIVCTFQEYAGRCGDKYKLCQAELKELLQKELATWTPTEFRECDYNKFMSVLDTNKDCEVDFVEYVRSLACLCLYCHEYFKDCPSEPPCSQ

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A3
Calculated molecular weight 12 kDa
Observed molecular weight 7 kDa, 12 kDa
GenBank accession numberBC012893
Gene Symbol S100A3
Gene ID (NCBI) 6274
RRIDAB_2183761
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP33764
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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