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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100A2 Polyclonal antibody (15454-1-AP)

    WB analysis using 15454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15454-1-AP (S100A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A2 Polyclonal antibody (15454-1-AP)

    WB analysis using 15454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15454-1-AP (S100A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using S100A2 Polyclonal antibody (15454-1-AP)

    IHC staining of human bowen disease using 15454-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15454-1-AP (S100A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using S100A2 Polyclonal antibody (15454-1-AP)

    IHC staining of human bowen disease using 15454-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15454-1-AP (S100A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using S100A2 Polyclonal antibody (15454-1-AP)

    IF Staining of A431 using 15454-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using S100A2 antibody (15454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using S100A2 Polyclonal antibody (15454-1-AP)

    FC experiment of A431 using 15454-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug S100A2 Polyclonal antibody (15454-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

S100A2 Polyclonal antibody

S100A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15454-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CAN19, Protein S 100L, Protein S100 A2, Protein S100-A2, Protein S-100L

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHaCaT cells, HeLa cells
Positive IHC detected inhuman bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15454-1-AP targets S100A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7719

Product name: Recombinant human S100A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-98 aa of BC002829

Sequence: MMCSSLEQALAVLVTTFHKYSCQEGDKFKLSKGEMKELLHKELPSFVGEKVDEEGLKKLMGSLDENSDQQVDFQEYAVFLALITVMCNDFFQGCPDRP

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A2
Calculated molecular weight 11 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC002829
Gene Symbol S100A2
Gene ID (NCBI) 6273
RRIDAB_3669209
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29034
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A2 (S100 calcium-binding protein A2) is also named as S100L and CAN19. S100A2, a member of the S100 protein family, is more highly expressed in endometrial carcinoma tissues than in normal tissues and was correlated with worse survival (PMID: 35042454). S100A2 has a tumor suppressor function. S100A2 is highly upregulated in the epidermis under inflammatory conditions and in drug eruptions, in addition to inflammatory skin diseases such as atopic dermatitis and psoriasis (PMID: 35885660).

Protocols

Product Specific Protocols
FC protocol for S100A2 antibody 15454-1-APDownload protocol
IF protocol for S100A2 antibody 15454-1-APDownload protocol
IHC protocol for S100A2 antibody 15454-1-APDownload protocol
WB protocol for S100A2 antibody 15454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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