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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100A16 Polyclonal antibody (11456-1-AP)

    WB analysis using 11456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using S100A16 Polyclonal antibody (11456-1-AP)

    WB analysis of A431 using 11456-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using S100A16 Polyclonal antibody (11456-1-AP)

    WB analysis of MCF-7 using 11456-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IP experiment of mouse brain using 11456-1-AP

    IP result of anti-S100A16 (IP:11456-1-AP, 4ug; Detection:11456-1-AP 1:2000) with mouse brain tissue lysate 1680 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of mouse lung using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human bowen disease using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human bowen disease using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human bowen disease using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S100A16 Polyclonal antibody (11456-1-AP)

    IF Staining of MCF-7 using 11456-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using S100A16 antibody (11456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

S100A16 Polyclonal antibody

S100A16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11456-1-AP
Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

AAG13, Aging-associated gene 13 protein, Protein S100 A16, Protein S100 F, Protein S100-A16

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, DU 145 cells, MCF-7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human bowen disease, human colon cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11456-1-AP targets S100A16 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2023

Product name: Recombinant human S100A16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-103 aa of BC019099

Sequence: MSDCYTELEKAVIVLVENFYKYVSKYSLVKNKISKSSFREMLQKELNHMLSDTGNRKAADKLIQNLDANHDGRISFDEYWTLIGGITGPIAKLIHEQEQQSSS

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A16
Calculated molecular weight 103 aa, 12 kDa
Observed molecular weight 9-12 kDa
GenBank accession numberBC019099
Gene Symbol S100A16
Gene ID (NCBI) 140576
RRIDAB_2269940
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

Protocols

Product Specific Protocols
IF protocol for S100A16 antibody 11456-1-APDownload protocol
IHC protocol for S100A16 antibody 11456-1-APDownload protocol
IP protocol for S100A16 antibody 11456-1-APDownload protocol
WB protocol for S100A16 antibody 11456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat,mouseWB

Metabolism

S100A16 mediation of weight gain attenuation induced by dietary calcium.

Authors - Zhang Rihua R
View Article
mouseWB,IHC,IF

Cell Death Dis

Interaction of calcium binding protein S100A16 with myosin-9 promotes cytoskeleton reorganization in renal tubulointerstitial fibrosis.

Authors - Hui Sun
View Article
humanWB

Cancer Lett

Integrative single-cell and spatial transcriptomic reveals S100A16(+) tumor endothelial cells drive angiogenesis and immunosuppression in hepatocellular carcinoma.

Authors - Xiang-Xu Wang
  • KD Validated
View Article
humanWB,IHC,IF

Int J Biol Sci

S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development

Authors - Yifang Hu
  • KD Validated
View Article

J Ethnopharmacol

Digeda-8 formula attenuates cholestatic liver injury in rats by restoring bile acid transport and suppressing inflammation: An integrated metabolomics and proteomics approach.

Authors - Alamusi Bayoude
View Article
mouseWB,IF

Cell Mol Life Sci

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Authors - Yifei Sun
  • KO Validated
View Article
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