Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, rat stomach tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue, human skin tissue, human ovary tumor tissue, human breast cancer tissue, human bowen disease, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 23 publications below |
| IHC | See 19 publications below |
| IF | See 7 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
10489-1-AP targets S100A14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0757 Product name: Recombinant human S100A14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-104 aa of BC005019 Sequence: MGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKETLTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGEAAKSVKLERPVRGH 相同性解析による交差性が予測される生物種 |
| Full Name | S100 calcium binding protein A14 |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 10-12 kDa |
| GenBank accession number | BC005019 |
| Gene Symbol | S100A14 |
| Gene ID (NCBI) | 57402 |
| RRID | AB_2183628 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for S100A14 antibody 10489-1-AP | Download protocol |
| IHC protocol for S100A14 antibody 10489-1-AP | Download protocol |
| IP protocol for S100A14 antibody 10489-1-AP | Download protocol |
| WB protocol for S100A14 antibody 10489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Senolytic-sensitive p16(Ink4a)+ fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity. | ||
Mol Cell hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-κB p50 | ||
Cell Death Dis S100A14 promotes colorectal cancer progression and anti-PD-1 resistance via UPF1-mediated activation of the non-canonical NF-κB signaling. | ||
Cell Death Dis Calcium-binding protein S100A14 induces differentiation and suppresses metastasis in gastric cancer. | ||
Cancer Lett Disruption of enhancer-driven S100A14 expression promotes esophageal carcinogenesis | ||
Cell Death Discov CAV2 promotes the invasion and metastasis of head and neck squamous cell carcinomas by regulating S100 proteins |