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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100A1 Recombinant monoclonal antibody (88164-1-RR)

    WB analysis using 88164-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88164-1-RR (S100A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Recombinant monoclonal antibody (88164-1-RR)

    IHC staining of human tonsillitis using 88164-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88164-1-RR (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Recombinant monoclonal antibody (88164-1-RR)

    IHC staining of human tonsillitis using 88164-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88164-1-RR (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using S100A1 Recombinant monoclonal antibody (88164-1-RR)

    IF Staining of A375 using 88164-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using S100A1 antibody (88164-1-RR, Clone: 252961A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

S100A1 Recombinant monoclonal antibody

S100A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 88164-1-RR
Clone No.252961A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein S100 A1, Protein S100-A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100 calcium-binding protein A1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88164-1-RR targets S100A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8871

Product name: Recombinant human S100A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-94 aa of BC014392

Sequence: MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A1
Calculated molecular weight 94 aa, 11 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC014392
Gene Symbol S100A1
Gene ID (NCBI) 6271
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A1 belongs to the S100 protein family, which represents the largest group among EF-hand Ca2+-binding proteins exclusively expressed in vertebrates. S100 proteins have been described as central regulators of numerous cellular and molecular processes, often operating in a cell-type-specific manner (PMID: 11991838). S100A1 is a central regulator of Ca2+-driven networks that orchestrates compartmentalized Ca2+ fluxes in the heart (PMID: 22336719, PMID: 26021637). S100A1 is primarily expressed in cardiomyocytes (CM). Endothelial cells (EC) express significantly lower S100A1 levels, whereas fibroblasts do not express S100A1 (PMID: 38641766).

Protocols

Product Specific Protocols
IF protocol for S100A1 antibody 88164-1-RRDownload protocol
IHC protocol for S100A1 antibody 88164-1-RRDownload protocol
WB protocol for S100A1 antibody 88164-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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