Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
Product Information
27865-1-AP targets RyDEN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26838 Product name: Recombinant human C19orf66 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC035817 Sequence: MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPEDMKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPADKMWGLAEFHCPKCRHNFRTHTHSCSAADCYNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEEEVEDEEGGPRE 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 19 open reading frame 66 |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC035817 |
| Gene Symbol | C19orf66 |
| Gene ID (NCBI) | 55337 |
| RRID | AB_2880997 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUL5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The shiftless antiviral inhibitor of ribosomal frameshifting (SHFL) gene was firstly predicted as an ISG in 2011, which is also named as C19orf66, FLJ11286, IRAV, UPF0515, and RyDEN. SHFL showed high expressing level in human liver and spleen tissues, whereas a lower expression is observed in the pancreas and heart. SHFL is a novel ISG that inhibits dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) infections. Interestingly, SHFL functions as a broad-spectrum antiviral factor exhibiting suppressive activity against various types of RNA and DNA viruses. (PMID: 36293480, PMID: 26735137, PMID: 38149274)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RyDEN antibody 27865-1-AP | Download protocol |
| WB protocol for RyDEN antibody 27865-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting. | ||
Proc Natl Acad Sci U S A Shiftless inhibits flavivirus replication in vitro and is neuroprotective in a mouse model of Zika virus pathogenesis.
| ||
Int Immunopharmacol C19orf66 restricts Coxsackievirus B infection by inducing lysosomal degradation of the viral proteins 3Dpol and 2Apro and exhibits neuroprotective effects in CVB-challenged mice | ||
J Virol Porcine reproductive and respiratory syndrome virus antagonizes the host restriction factor SHFL to sustain viral programmed ribosomal frameshifting and replication.
| ||
PLoS Pathog SAMD9L inhibits flavivirus translation independently of its capacity to trigger innate immune response. |