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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Resistin Polyclonal antibody (32786-1-AP)

    WB analysis using 32786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32786-1-AP (Resistin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. Mouse lung tissue as the negative control.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Resistin Polyclonal antibody

Resistin Polyclonal Antibody for WB, ELISA
Cat No. 32786-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, ADSF, Cysteine-rich secreted protein FIZZ3, Fizz3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse adipose tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32786-1-AP targets Resistin in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Resistin fusion protein Eg3391 相同性解析による交差性が予測される生物種
Full Name resistin
Calculated molecular weight12 kDa
Observed molecular weight12 kDa
GenBank accession numberNM_001204959.1
Gene Symbol Retn
Gene ID (NCBI) 57264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ99P87
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Resistin, an adipokine produced in fat tissue, is a cysteine rich protein with a molecular weight of 12 kDa. Resistin inhibits insulin action, signaling, and glucose metabolism. Previous data have suggested that resistin secretion is linked to insulin resistance (IR) and type 2 diabetes. Resistin expression in rodents is limited only to adipocytes, but human resistin is produced mainly by macrophages and monocytes, which show only 59% amino acid homology to mice. (PMID: 34325643, PMID: 32991315)

Protocols

Product Specific Protocols
WB protocol for Resistin antibody 32786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols