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Validation Data Gallery

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  • Western Blot (WB) analysis of pig retina tissue using Recoverin Monoclonal antibody (66521-1-Ig)

    WB analysis of pig retina using 66521-1-Ig (same clone as 66521-1-PBS)

    pig retina tissue were subjected to SDS PAGE followed by western blot with 66521-1-Ig (Recoverin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66521-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat retina tissue using Recoverin Monoclonal antibody (66521-1-Ig)

    WB analysis of rat retina using 66521-1-Ig (same clone as 66521-1-PBS)

    rat retina tissue were subjected to SDS PAGE followed by western blot with 66521-1-Ig (Recoverin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66521-1-PBS in a different storage buffer formulation.

Recoverin Monoclonal antibody, PBS Only

Recoverin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66521-1-PBS
Clone No.3B8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig, mouse

Applications

WB, Indirect ELISA

Synonyms

Protein CAR, RCV1, RCVRN, recoverin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66521-1-PBS targets Recoverin in WB, Indirect ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Reactivity Human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0119

Product name: Recombinant human Recoverin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-166 aa of BC001720

Sequence: GALSKEILEELQLNTKFSEEELCSWYQSFLKDCPTGRITQQQFQSIYAKFFPDTDPKAYAQHVFRSFDSNLDGTLDFKEYVIALHMTTAGKTNQKLEWAFSLYDVDGNGTISKNEVLEIVMAIFKMITPEDVKLLPDDENTPEKRAEKIWKYFGKNDDDK

相同性解析による交差性が予測される生物種
Full Name recoverin
Calculated molecular weight 24 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001720
Gene Symbol Recoverin
Gene ID (NCBI) 5957
RRIDAB_2881884
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP35243
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.