Recombinant Rat NGFR/TNFRSF16 protein (rFc Tag)(HPLC verified)
Species
Rat
Purity
>90 %, SDS-PAGE
>90 %, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg3254
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat p75NTR protein Lys30-Ile253 (Accession# P07174) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 24596 |
| Accession | P07174 |
| PredictedSize | 50.2 kDa |
| SDS-PAGE | 65-90 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
p75 neurotrophin receptor (p75NTR), a member of the tumor necrosis factor superfamily, is a transmembrane protein with 75 kDa molecular weight. p75NTR is highly expressed in both neurons and glial cells during CNS development and after brain injury, whereas in normal adulthood, it is present only in specific brain regions such as neurons and astrocytes of hippocampal and especially in the cholinergic neurons of the basal forebrain. p75NTR is an important receptor for the role of neurotrophins in survival and death of neurons during development and after nerve injury. p75NTR can mediate cell survival or cell death, can promote or inhibit axonal growth, and can facilitate or attenuate proliferation, depending on the cell context.
References:
1.Cragnolini AB and Friedman WJ. (2008) Trends Neurosci. 31(2):99-104. 2.Chen M. et al. (2022) Cell Mol Neurobiol. 42(4):1153-1166. 3.Yang CR. et al. (2022) FASEB J. 36(3):e22180. 4. Ali NH. Et al. (2024) Mol Neurobiol. 61(5):2469-2480. 5. Xie B. et al. (2023) Front Cell Dev Biol. 11:1283878.