Speedy™ Rat IL-6 One-Step ELISA Kit
Sensitivity
11.5 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Rat
Cat no : SE70002
Synonyms
IL6, Ifnb2, IL 6, ILg6, interleukin 6
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat IL-6 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 11.5 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24498 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 91% | 73%-119% |
| Cell culture supernatant | 91% | 85%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,099.6 | 29.9 | 1.4 |
| 2 | 8 | 519.9 | 11.6 | 2.2 |
| 3 | 8 | 271.9 | 5.6 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,091.1 | 32.9 | 1.6 |
| 2 | 16 | 521.2 | 10.2 | 1.9 |
| 3 | 16 | 273.1 | 7.7 | 2.8 |
Background Information
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL6, Ifnb2, IL 6, ILg6, interleukin 6 |