Rat IL-4 ELISA Kit
Cat no : KE20038
Synonyms
B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, Il4
Validation Data Gallery
Product Information
KE20038 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL-4 ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL-4. The assay recognizes rat IL-4. An antibody specific for rat IL-4 has been pre-coated onto the microwells. The rat IL-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat IL-4 is added to detect the captured rat IL-4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 11.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 287287 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 87% | 82%-93% |
| Cell culture supernatant | 103% | 101%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,149.8 | 33.7 | 2.9 |
| 2 | 8 | 278.4 | 11.7 | 4.2 |
| 3 | 8 | 146.6 | 7.6 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,135.2 | 40.7 | 3.6 |
| 2 | 16 | 279.7 | 14.6 | 5.2 |
| 3 | 16 | 143.0 | 8.2 | 5.8 |
Background Information
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, Il4 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Ethnopharmacol Exploring the dosage-effect relationship and mechanism of Huangqi in Huangqi Guizhi Wuwu decoction for treating rheumatoid arthritis based on the compatibility of the formula. | ||
Front Pharmacol Investigating the regulation of the miR-199a-3p/TGF-β/Smad signaling pathway by BSHXF drug-containing serum combined with ADSCs for delaying intervertebral disc degeneration |