Recombinant Rat IL-1 beta protein (rFc Tag)
Species
Rat
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2582
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat IL-1 beta protein Val117-Ser268 (Accession# Q63264) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 24494 |
| Accession | Q63264 |
| PredictedSize | 43.6 kDa |
| SDS-PAGE | 43-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL1 gene family encodes three proteins: IL1 -alpha IL1- beta and their naturally occurring inhibitor IL1-RN. Interleukin 1 beta(IL1- beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL1-beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL1-beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
References:
1. Dinarello CA. et al. (1996). Blood. 87: 2095-147. 2. Bird S. et al. (2002). Cytokine Growth Factor Rev. 13: 483-502. 3. Xu J. et al. (2013). PLoS One. 21;8(5):e63654. 4. McCarty S. (2014). Cardiol Rev. 22: 176-81.