Recombinant Rat IFN-gamma R1/CD119 protein (rFc Tag)
Species
Rat
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4478
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat IFN-gamma R1 protein Ala17-Ser241 (Accession# Q9QZ62) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 116465 |
| Accession | Q9QZ62 |
| PredictedSize | 51.5 kDa |
| SDS-PAGE | 60-85 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection.
References:
1. Huang CK, Chen Z, Zhou Z, et al. (2024) Circ Res. 135(4):518-536. 2. Du W, Hua F, Li X, et al. (2021) Cancer Discov. 11(7):1826-1843. 3. Soula M, Unlu G, Welch R, et al. (2024) Nature. 633(8029):451-458. 4. Otaki N, Motomura Y, Terooatea T, et al. (2023) Nat Commun. 14(1):8120.