Speedy™ Rat C-Reactive Protein/CRP One-Step ELISA Kit
Sensitivity
10.2 pg/mL
Range
39-2500 pg/mL
Reactivity
Rat
Cat no : SE70005
Synonyms
C-Reactive Protein, CRP, PTX 1, PTX1
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat C-Reactive Protein/CRP One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 10.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25419 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 103% | 99%-111% |
| Cell culture supernatant | 107% | 93%-127% |
| Urine | 116% | 105%-127% |
| Tissue lysate | 92% | 89%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,344.6 | 57.2 | 4.3 |
| 2 | 8 | 338.2 | 5.1 | 1.5 |
| 3 | 8 | 188.8 | 4.5 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,327.6 | 65.2 | 4.9 |
| 2 | 16 | 339.5 | 8.2 | 2.4 |
| 3 | 16 | 187.6 | 5.7 | 3.0 |
Background Information
C-reactive protein (CRP) is an evolutionary highly conserved member of the pentraxin superfamily of proteins. CRP consists of five identical subunits with molecular weight 20-30 kDa arranged as a cyclic pentamer. CRP exists in at least two conformationally distinct forms, native pentameric CRP (pCRP) and modified/monomeric CRP (mCRP). CRP plays important roles in inflammatory processes and host responses to infection including promoting agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its Ca2+-dependent binding to phosphorylcholine (PCh). CRP is mainly expressed by hepatocytes and secreted into plasma. The concentration of CRP will increase significantly in plasma during acute phase response to tissue injury, infection, or other inflammatory stimuli.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-Reactive Protein, CRP, PTX 1, PTX1 |