Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, HEK-293 cells, mouse brain tissue, fetal human brain tissue, HeLa cells, RAW 264.7 cells, NIH/3T3 cells, 3T3-L1 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 3 publications below |
Product Information
27804-1-AP targets RanBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25714 Product name: Recombinant human RANBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: MLNAENAQKFKTKFEECRKEIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ 相同性解析による交差性が予測される生物種 |
| Full Name | RAN binding protein 1 |
| Observed molecular weight | 28 kDa |
| Gene Symbol | RANBP1 |
| Gene ID (NCBI) | 5902 |
| RRID | AB_2880977 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43487 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RanBP1 antibody 27804-1-AP | Download protocol |
| IHC protocol for RanBP1 antibody 27804-1-AP | Download protocol |
| WB protocol for RanBP1 antibody 27804-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Chem Structure-Guided Design of the First Noncovalent Small-Molecule Inhibitor of CRM1. | ||
Biomolecules Mutations of Key Functional Residues in CRM1/XPO1 Differently Alter Its Intranuclear Localization and the Nuclear Export of Endogenous Cargos | ||
J Transl Med Targeting deubiquitinase USP7-mediated stabilization of XPO1 contributes to the anti-myeloma effects of selinexor | ||
Elife Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals. |
