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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RanBP1 Polyclonal antibody

RanBP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27804-1-AP

Synonyms

HTF9A, RAN binding protein 1, RANBP1

Validation Data Gallery

Applications:
  • WB analysis using 27804-1-AP

    WB analysis using 27804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27804-1-AP

    WB analysis using 27804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 27804-1-AP

    WB analysis of fetal human brain using 27804-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 27804-1-AP

    WB analysis of mouse brain using 27804-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 27804-1-AP

    IHC staining of mouse testis using 27804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27804-1-AP (RanBP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RANBP1 antibody included
  • IF Staining of HeLa using 27804-1-AP

    IF Staining of HeLa using 27804-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 27804-1-AP

    IF Staining of NIH/3T3 using 27804-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells, mouse brain tissue, fetal human brain tissue, HeLa cells, RAW 264.7 cells, NIH/3T3 cells, 3T3-L1 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RANBP1 Ready-To-Use IHC Kit. RANBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27804-1-AP targets RanBP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RanBP1 fusion protein Ag25714 相同性解析による交差性が予測される生物種
Full Name RAN binding protein 1
Observed molecular weight 28 kDa
Gene symbol RANBP1
Gene ID (NCBI) 5902
RRIDAB_2880977
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).

Protocols

Product Specific Protocols
WB protocol for RanBP1 antibody 27804-1-APDownload protocol
IHC protocol for RanBP1 antibody 27804-1-APDownload protocol
IF protocol for RanBP1 antibody 27804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Med Chem

Structure-Guided Design of the First Noncovalent Small-Molecule Inhibitor of CRM1.

Authors - Yuqin Lei
View Article
humanWB

Elife

Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.

Authors - Yuling Li
View Article