Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 22218-1-AP (same clone as 22218-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 22218-1-AP (same clone as 22218-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 22218-1-AP (same clone as 22218-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 22218-1-AP (same clone as 22218-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 22218-1-AP (same clone as 22218-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 22218-1-AP (same clone as 22218-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 22218-1-AP (same clone as 22218-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 22218-1-AP (same clone as 22218-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
IP experiment of mouse heart using 22218-1-AP (same clone as 22218-1-PBS)
IP result of anti-Rabenosyn 5 (IP:22218-1-AP, 4ug; Detection:22218-1-AP 1:10000) with mouse heart tissue lysate 1920 ug. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 22218-1-AP (same clone as 22218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 22218-1-AP (same clone as 22218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 22218-1-AP (same clone as 22218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 22218-1-AP (same clone as 22218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

Rabenosyn 5 Polyclonal antibody, PBS Only

Rabenosyn 5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 22218-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ZFYVE20, 110 kDa protein, FYVE finger-containing Rab5 effector protein rabenosyn-5, Rabenosyn-5, RBSN

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22218-1-PBS targets Rabenosyn 5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17554 Product name: Recombinant human ZFYVE20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 437-784 aa of BC106940 Sequence: LRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLRQLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLEPSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELKHTLAKQKGGTD 相同性解析による交差性が予測される生物種
Full Name	zinc finger, FYVE domain containing 20
Calculated molecular weight	784 aa, 89 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC106940
Gene Symbol	Rabenosyn 5
Gene ID (NCBI)	64145
RRID	AB_11182179
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1K0
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of K-562 using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of mouse testis using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of mouse brain using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of A549 using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of mouse skeletal muscle using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of mouse heart using 22218-1-AP (same clone as 22218-1-PBS)

WB analysis of mouse kidney using 22218-1-AP (same clone as 22218-1-PBS)

IP experiment of mouse heart using 22218-1-AP (same clone as 22218-1-PBS)

IHC staining of human testis using 22218-1-AP (same clone as 22218-1-PBS)