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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RWDD4A Polyclonal antibody (32101-1-AP)

    WB analysis using 32101-1-AP

    5637 cells were subjected to SDS PAGE followed by western blot with 32101-1-AP (RWDD4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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RWDD4A Polyclonal antibody

RWDD4A Polyclonal Antibody for WB, ELISA
Cat No. 32101-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RWDD4, RWD domain-containing protein 4, Protein FAM28A, FAM28A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in5637 cells, SH-SY5Y cells, U-251 cells, U-87MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32101-1-AP targets RWDD4A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RWDD4A fusion protein Ag36777 相同性解析による交差性が予測される生物種
Full Name RWD domain containing 4A
Calculated molecular weight21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC107432
Gene Symbol RWDD4A
Gene ID (NCBI) 201965
RRIDAB_3670191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6NW29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RWDD4, which is a poorly characterized gene that encodes the protein RWD Domain Containing 4, with an RWD domain being involved in protein-protein interactions (PMID: 27916600). RWDD4 was revealed to be a miR506 target in BCa cells, and the downregulation of RWDD4 was found to suppress BCa cell proliferation, migration and invasion (PMID: 30655740).

Protocols

Product Specific Protocols
WB protocol for RWDD4A antibody 32101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols