• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUVBL2 Monoclonal antibody

RUVBL2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 67851-1-Ig

Synonyms

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

Validation Data Gallery

Applications:
  • WB analysis using 67851-1-Ig

    WB analysis using 67851-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67851-1-Ig (RUVBL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung cancer using 67851-1-Ig

    IHC staining of human lung cancer using 67851-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of human lung cancer using 67851-1-Ig

    IHC staining of human lung cancer using 67851-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IF Staining of HeLa using 67851-1-Ig

    IF Staining of HeLa using 67851-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RUVBL2 antibody (67851-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RUVBL2 Ready-To-Use IHC Kit. RUVBL2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67851-1-Ig targets RUVBL2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RUVBL2 fusion protein Ag0253 相同性解析による交差性が予測される生物種
Full Name RuvB-like 2 (E. coli)
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC000428
Gene symbol RUVBL2
Gene ID (NCBI) 10856
RRIDAB_2918610
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)

Protocols

Product Specific Protocols
WB protocol for RUVBL2 antibody 67851-1-IgDownload protocol
IHC protocol for RUVBL2 antibody 67851-1-IgDownload protocol
IF protocol for RUVBL2 antibody 67851-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols