Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, SGC-7901 cells, HeLa cells, BGC-823 cells, mouse brain tissue, rat brain tissue |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83601-2-RR targets RUFY3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35558 Product name: Recombinant human RUFY3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-469 aa of BC051716 Sequence: HCLKHGLKAKKTFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMKGEDLDSQVGVIDFSMYLKDGNSSKGTEGDGQITAILDQKNYVEELNRHLNATVNNLQAKVDALEKSNTKLTEELAVANNRIITLQEEMERVKEESSYILESNRKGPKQDRTAEGQALSEARKHLKEETQLRLDVEKELEMQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH 相同性解析による交差性が予測される生物種 |
| Full Name | RUN and FYVE domain containing 3 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC051716 |
| Gene Symbol | RUFY3 |
| Gene ID (NCBI) | 22902 |
| RRID | AB_3671215 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7L099 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RUFY3 (RUN and FYVE domain-containing protein 3) was originally described as lacking part of the C-terminal CC2 and FYVE domains. The interaction of RUFY3 with the filamentous actin network through a complex formed together with Rap2 and Fascin 1 (FSCN1) is critical for axonogenesis and growth cone development (PMID: 37463962). RUFY3 also seems to play a crucial role in promoting cancer development (PMID: 34510031).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RUFY3 antibody 83601-2-RR | Download protocol |
| WB protocol for RUFY3 antibody 83601-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |