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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTN3 Polyclonal antibody (12055-2-AP)

    WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RTN3 Polyclonal antibody (12055-2-AP)

    WB analysis of human brain using 12055-2-AP (same clone as 12055-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RTN3 Polyclonal antibody (12055-2-AP)

    WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RTN3 Polyclonal antibody (12055-2-AP)

    IP experiment of HeLa using 12055-2-AP (same clone as 12055-2-PBS)

    IP result of anti-RTN3 (IP:12055-2-AP, 4ug; Detection:12055-2-AP 1:400) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using RTN3 Polyclonal antibody (12055-2-AP)

    IHC staining of rat brain using 12055-2-AP (same clone as 12055-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12055-2-AP (RTN3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using RTN3 Polyclonal antibody (12055-2-AP)

    IHC staining of rat brain using 12055-2-AP (same clone as 12055-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12055-2-AP (RTN3 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RTN3 Polyclonal antibody (12055-2-AP)

    IF Staining of mouse brain using 12055-2-AP (same clone as 12055-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using RTN3 antibody (12055-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

RTN3 Polyclonal antibody, PBS Only

RTN3 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 12055-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

ASYIP, HAP, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12055-2-PBS targets RTN3 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2685

Product name: Recombinant human RTN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-236 aa of BC011394

Sequence: MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE

相同性解析による交差性が予測される生物種
Full Name reticulon 3
Calculated molecular weight 113 kDa
Observed molecular weight 24 kDa, 100-113 kDa
GenBank accession numberBC011394
Gene Symbol RTN3
Gene ID (NCBI) 10313
RRIDAB_2301357
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95197
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

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