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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    WB analysis of mouse skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    WB analysis of human skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    WB analysis of mouse skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    WB analysis of mouse skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    WB analysis of human skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    IHC staining of human skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11168-1-AP (RTN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using RTN2 Polyclonal antibody (11168-1-AP)

    IHC staining of human skeletal muscle using 11168-1-AP (same clone as 11168-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11168-1-AP (RTN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11168-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RTN2 Polyclonal antibody, PBS Only

RTN2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11168-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11168-1-PBS targets RTN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1655

Product name: Recombinant human RTN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 344-545 aa of BC014244

Sequence: KVADLLYWKDTRTSGVVFTGLMVSLLCLLHFSIVSVAAHLALLLLCGTISLRVYRKVLQAVHRGDGANPFQAYLDVDLTLTREQTERLSHQITSRVVSAATQLRHFFLVEDLVDSLKLALLFYILTFVGAIFNGLTLLILGVIGLFTIPLLYRQHQAQIDQYVGLVTNQLSHIKAKIRAKIPGTGALASAAAAVSGSKAKAE

相同性解析による交差性が予測される生物種
Full Name reticulon 2
Calculated molecular weight 59 kDa, 51 kDa, 22 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC014244
Gene Symbol RTN2
Gene ID (NCBI) 6253
RRIDAB_10644321
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75298
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. The human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.

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