Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HL-60 using 17458-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17458-1-AP
WB result of RTKN2 antibody (17458-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RTKN2 transfected HEK-293 cells.
WB analysis of HEK-293 using 17458-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 17458-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lymph using 17458-1-AP
mouse lymph tissue were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17458-1-AP (RTKN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17458-1-AP (RTKN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 17458-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 17458-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RTKN2 antibody (17458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

RTKN2 Polyclonal antibody

RTKN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17458-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PH domain-containing family K member 1, Pleckstrin homology domain-containing family K member 1, PLEKHK1, rhotekin 2, Rhotekin-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, HEK-293 cells, mouse lymph tissue
Positive IHC detected in	human placenta tissue, human brain tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below

Product Information

17458-1-AP targets RTKN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11098 Product name: Recombinant human RTKN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-163 aa of BC025765 Sequence: MEGPSLRGPALRLAGLPTQQDCNIQEKIDLEIRMREGIWKLLSLSTQKDQVLHAVKNLMVCNARLMAYTSELQKLEEQIANQTGRCDVKFESKERTACKGKIAISDIRIPLMWKDSDHFSNKERSRRYAIFCLFKMGANVFDTDVVNVDKTITDICFENVTIL 相同性解析による交差性が予測される生物種
Full Name	rhotekin 2
Calculated molecular weight	163aa,19 kDa; 609aa,69 kDa
Observed molecular weight	69 kDa
GenBank accession number	BC025765
Gene Symbol	RTKN2
Gene ID (NCBI)	219790
RRID	AB_2034939
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IZC4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RTKN2 antibody 17458-1-AP	Download protocol
IHC protocol for RTKN2 antibody 17458-1-AP	Download protocol
WB protocol for RTKN2 antibody 17458-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Rep Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity. Authors - Brendan Gongol KD Validated View Article
human	WB,IHC	J Viral Hepat Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway Authors - Li Zhang View Article
human	WB	Sci Rep RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway Authors - Xiaomei Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HL-60 using 17458-1-AP

WB analysis of HEK-293 using 17458-1-AP

WB analysis of HEK-293 using 17458-1-AP

WB analysis of Raji using 17458-1-AP

WB analysis of mouse lymph using 17458-1-AP

IHC staining of human placenta using 17458-1-AP

IHC staining of human placenta using 17458-1-AP

IHC staining of human placenta using 17458-1-AP

IHC staining of human placenta using 17458-1-AP

IHC staining of human skin using 17458-1-AP

IHC staining of human skin using 17458-1-AP

IHC staining of human brain using 17458-1-AP

IHC staining of human brain using 17458-1-AP

IF Staining of HEK-293 using 17458-1-AP

RTKN2 Polyclonal antibody

RTKN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity.

Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway

RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway