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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTF2 Polyclonal antibody (16633-1-AP)

    WB analysis using 16633-1-AP (same clone as 16633-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16633-1-AP (RTF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16633-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RTF2 Polyclonal antibody (16633-1-AP)

    WB analysis of HeLa using 16633-1-AP (same clone as 16633-1-PBS)

    WB result of RTF2 antibody (16633-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RTF2 transfected HeLa cells. This data was developed using the same antibody clone with 16633-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RTF2 Polyclonal antibody (16633-1-AP)

    IP experiment of HeLa using 16633-1-AP (same clone as 16633-1-PBS)

    IP result of anti-RTF2 (IP:16633-1-AP, 3ug; Detection:16633-1-AP 1:500) with HeLa cells lysate 3440ug. This data was developed using the same antibody clone with 16633-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RTF2 Polyclonal antibody (16633-1-AP)

    IF Staining of HeLa using 16633-1-AP (same clone as 16633-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RTF2 antibody (16633-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16633-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RTF2 Polyclonal antibody, PBS Only

RTF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 16633-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

C20orf43, AD-007, CDAO5, HSPC164, HSPC169

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16633-1-PBS targets RTF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9972

Product name: Recombinant human C20orf43 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of BC003359

Sequence: MGCDGGTIPKRHELVKGPKKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKAASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLRCCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKKTKKPKAAESVSKPDVSEEAPGPSKVKTGKPEEASLDSREKKTNLAPKSTAMNESSSGKAGKPPCGATKRSIADSEESEAYKSLFTTHSSAKRSKEESAHWVTHTSYCF

相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 43
Calculated molecular weight 34 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC003359
Gene Symbol RTF2
Gene ID (NCBI) 51507
RRIDAB_2256547
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY42
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The function of C20orf43 remains largely unknown. Catalog#16633-1-AP is a rabbit polyclonal antibody raised against the full-length of human C20orf43. The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.

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