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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis using 12170-1-AP (same clone as 12170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of HL-60 using 12170-1-AP (same clone as 12170-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of rat brain using 12170-1-AP (same clone as 12170-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of mouse brain using 12170-1-AP (same clone as 12170-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RTF1 Polyclonal antibody (12170-1-AP)

    IP experiment of HeLa using 12170-1-AP (same clone as 12170-1-PBS)

    IP result of anti-RTF1 (IP:12170-1-AP, 4ug; Detection:12170-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RTF1 Polyclonal antibody (12170-1-AP)

    IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RTF1 Polyclonal antibody (12170-1-AP)

    IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

RTF1 Polyclonal antibody, PBS Only

RTF1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12170-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CDG1N, GTL7, KIAA0252, RNA polymerase-associated protein RTF1 homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12170-1-PBS targets RTF1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2812

Product name: Recombinant human RTF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-307 aa of BC015052

Sequence: MKKQANKTASSGSSDKDSSAESSAPEEGEVSDSDSNSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEEDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGN

相同性解析による交差性が予測される生物種
Full Name Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight585aa,67 kDa; 710aa,80 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC015052
Gene Symbol RTF1
Gene ID (NCBI) 23168
RRIDAB_2180931
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92541
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RTF1 (RNA polymerase-associated protein RTF1 homolog) is a multifunctional component of the Paf1 complex that regulates gene expression by directing cotranscriptional histone modification (PMID: 17576814). RTF1 is essential for global methylation of H3-Lys4 and H3-Lys79, but not H3-Lys36 (PMID: 12876293).

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