Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of BGC-823 using 25337-1-AP
BGC-823 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 25337-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25337-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25337-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 25337-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25337-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 25337-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 25337-1-AP
IP result of anti-RTEL1 (IP:25337-1-AP, 4ug; Detection:25337-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IF Staining of HepG2 using 25337-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTEL1 antibody (25337-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RTEL1 Polyclonal antibody

RTEL1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 25337-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Novel helicase like, NHL, KIAA1088, EC:3.6.4.12, C20orf41

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	BGC-823 cells, C6 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, PC-3 cells
Positive IP detected in	mouse brain tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

25337-1-AP targets RTEL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	regulator of telomere elongation helicase 1
Calculated molecular weight	142 kDa
Observed molecular weight	133 kDa
GenBank accession number	NM_016434
Gene Symbol	RTEL1
Gene ID (NCBI)	51750
RRID	AB_2880034
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZ71
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RTEL1 antibody 25337-1-AP	Download protocol
IP protocol for RTEL1 antibody 25337-1-AP	Download protocol
WB protocol for RTEL1 antibody 25337-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
zebrafish	WB	Nucleic Acids Res Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing. Authors - Jingzhen Li View Article
human	WB	Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors Authors - Yahui Wang KD Validated View Article
human	WB	J Clin Invest CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes Authors - Nunziata Maio View Article
human	WB	J Cancer Res Clin Oncol RTEL1 is upregulated in gastric cancer and promotes tumor growth Authors - Chunyu Yang View Article
human	WB	Cell Rep A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons Authors - Benedikt V Hölbling View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of BGC-823 using 25337-1-AP

WB analysis of C6 using 25337-1-AP

WB analysis of HeLa using 25337-1-AP

WB analysis of mouse brain using 25337-1-AP

WB analysis of PC-3 using 25337-1-AP

WB analysis of HepG2 using 25337-1-AP

WB analysis of mouse testis using 25337-1-AP

IP experiment of mouse brain using 25337-1-AP

IF Staining of HepG2 using 25337-1-AP

RTEL1 Polyclonal antibody

RTEL1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes

RTEL1 is upregulated in gastric cancer and promotes tumor growth

A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons