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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RSRC1 Polyclonal antibody (23826-1-AP)

    WB analysis of HeLa using 23826-1-AP (same clone as 23826-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23826-1-AP (RSRC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RSRC1 Polyclonal antibody (23826-1-AP)

    WB analysis of HepG2 using 23826-1-AP (same clone as 23826-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23826-1-AP (RSRC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RSRC1 Polyclonal antibody (23826-1-AP)

    IHC staining of human small intestine using 23826-1-AP (same clone as 23826-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RSRC1 Polyclonal antibody (23826-1-AP)

    IHC staining of human small intestine using 23826-1-AP (same clone as 23826-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RSRC1 Polyclonal antibody (23826-1-AP)

    IHC staining of human liver cancer using 23826-1-AP (same clone as 23826-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RSRC1 Polyclonal antibody (23826-1-AP)

    IHC staining of human liver cancer using 23826-1-AP (same clone as 23826-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RSRC1 Polyclonal antibody (23826-1-AP)

    IF Staining of HepG2 using 23826-1-AP (same clone as 23826-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSRC1 antibody (23826-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23826-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RSRC1 Polyclonal antibody, PBS Only

RSRC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23826-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SRrp53, Serine/Arginine-related protein 53, BM-011, BM 011, Arginine/serine-rich coiled-coil protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23826-1-PBS targets RSRC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20604

Product name: Recombinant human RSRC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 172-334 aa of BC006982

Sequence: AGLEHLPPAEQAKARLQLVLEAAAKADEALKAKERNEEEAKRRKEEDQATLVEQVKRVKEIEAIESDSFVQQTFRSSKEVKKSVEPSEVKQATSTSGPASAVADPPSTEKEIDPTSIPTAIKYQDDNSLAHPNLFIEKADAEEKWFKRLIALRQERLMGSPVA

相同性解析による交差性が予測される生物種
Full Name arginine/serine-rich coiled-coil 1
Calculated molecular weight 334 aa, 39 kDa
Observed molecular weight 38 kDa, 50 kDa
GenBank accession numberBC006982
Gene Symbol RSRC1
Gene ID (NCBI) 51319
RRIDAB_2879329
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96IZ7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RSRC1, also named as SRRP53, is a novel SR-related protein of an apparent molecular mass of 53 kDa (45-53 kDa). It is isolated in a gene trap screen that identifies proteins which localize to the nuclear speckles. RSRC1 plays a rol in pre-mRNA splicing and constitutive.

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