Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse trachea tissue, rat trachea tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21931-1-AP targets RSPH4A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16551 Product name: Recombinant human RSPH4A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC106949 Sequence: MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEIT 相同性解析による交差性が予測される生物種 |
| Full Name | radial spoke head 4 homolog A (Chlamydomonas) |
| Calculated molecular weight | 716 aa, 81 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC106949 |
| Gene Symbol | RSPH4A |
| Gene ID (NCBI) | 345895 |
| RRID | AB_3669381 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TD94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RSPH4A, also known as RSHL3, belongs to the radial spoke family. RSPH4A is the component of the axonemal radial spoke head which plays an important role in ciliary motility. Mutations in RSPH4A cause primary ciliary dyskinesia 1, a disease arising from dysmotility of motile cilia and sperm(PMID: 19200523, 23798057).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPH4A antibody 21931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |