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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RSL24D1 Polyclonal antibody

RSL24D1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25190-1-AP

Synonyms

C15orf15, HRP L30 iso, L30, Ribosomal protein L24 like, RLP24, RPL24, RPL24L, RSL24D1, TVAS3

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 25190-1-AP

    WB analysis of HepG2 using 25190-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 25190-1-AP

    WB analysis of PC-3 using 25190-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 25190-1-AP

    WB analysis of HL-60 using 25190-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 25190-1-AP

    WB analysis of HepG2 using 25190-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25190-1-AP

    IF Staining of HepG2 using 25190-1-AP

    Immunofluorescent analysis of HepG2 cells using 25190-1-AP (RSL24D1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells, PC-3 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25190-1-AP is RSL24D1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSL24D1 fusion protein Ag18195 相同性解析による交差性が予測される生物種
Full Name ribosomal L24 domain containing 1
Calculated molecular weight 163 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC008409
Gene symbol RSL24D1
Gene ID (NCBI) 51187
RRIDAB_2879950
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RSL24D1 antibody 25190-1-APDownload protocol
IF protocol for RSL24D1 antibody 25190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.

Authors - Chunyang Ni
View Article
humanWB

RNA

Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing

Authors - Mason A McCool
View Article
humanIF

mBio

Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication

Authors - Maikke B Ohlson
View Article