Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HL-60 cells, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 3 publications below |
Product Information
25190-1-AP targets RSL24D1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RSL24D1 fusion protein Ag18195 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal L24 domain containing 1 |
| Calculated molecular weight | 163 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC008409 |
| Gene Symbol | RSL24D1 |
| Gene ID (NCBI) | 51187 |
| RRID | AB_2879950 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHA3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RSL24D1 (Ribosomal L24 Domain Containing 1) is a ribosomal protein that is primarily localized in the nucleolus and is involved in the biogenesis of the 60S ribosomal subunit. In mouse embryonic stem cells (ESCs), the expression level of RSL24D1 is high and significantly decreases during cell differentiation. The role of RSL24D1 in ribosome biogenesis includes facilitating proper protein docking in nucleolar and cytoplasmic precursor particles. After the completion of biogenesis, RSL24D1 dissociates from the particles and is thought to be replaced by ribosomal protein L24.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSL24D1 antibody 25190-1-AP | Download protocol |
| IF protocol for RSL24D1 antibody 25190-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly. | ||
mBio Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication | ||
Cell The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity | ||
Nat Cell Biol A programmed decline in ribosome levels governs human early neurodevelopment |