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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RSL1D1 Polyclonal antibody (27667-1-AP)

    WB analysis using 27667-1-AP (same clone as 27667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27667-1-AP (RSL1D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RSL1D1 Polyclonal antibody (27667-1-AP)

    WB analysis using 27667-1-AP (same clone as 27667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27667-1-AP (RSL1D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RSL1D1 Polyclonal antibody (27667-1-AP)

    WB analysis using 27667-1-AP (same clone as 27667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27667-1-AP (RSL1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RSL1D1 Polyclonal antibody (27667-1-AP)

    IHC staining of mouse colon using 27667-1-AP (same clone as 27667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27667-1-AP (RSL1D1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RSL1D1 Polyclonal antibody (27667-1-AP)

    IHC staining of mouse colon using 27667-1-AP (same clone as 27667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27667-1-AP (RSL1D1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

RSL1D1 Polyclonal antibody, PBS Only

RSL1D1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27667-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27667-1-PBS targets RSL1D1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24923

Product name: Recombinant human RSL1D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 269-490 aa of BC112228

Sequence: SNWDEATKRSLLNKKKKEARRKRRERNFEKQKERKKKRQQARKTASVLSKDDVAPESGDTTVKKPESKKEQTPEHGKKKRGRGKAQVKATNESEDEIPQLVPIGKKTPANEKVEIQKHATGKKSPAKSPNPSTPRGKKRKALPASETPKAAESETPGKSPEKKPKIKEEAVKEKSPSLGKKDARQTPKKPEAKFFTTPSKSVRKASHTPKKWPKKPKVPQST

相同性解析による交差性が予測される生物種
Full Name ribosomal L1 domain containing 1
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight55-70 kDa
GenBank accession numberBC112228
Gene Symbol RSL1D1
Gene ID (NCBI) 26156
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO76021
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RL1D1, Ribosomal L1 domain-containing protein 1, also named CSIG, a member of the universal ribosomal protein uL1 family. RL1D1 regulates cellular senescence through inhibition of PTEN translation. It acts as a pro-apoptotic regulator in response to DNA damage (PMID: 18678645, PMID: 22419112). RSL1D1 promoted the progression of colorectal cancer through RAN-mediated autophagy suppression. RSL1D1 can be detected 70 kDa (PMID:35013134).

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