Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | MCF-7 cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21877-1-AP targets RSK4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16299 Product name: Recombinant human RPS6KA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 629-745 aa of BC148802 Sequence: TPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 6 |
| Calculated molecular weight | 745 aa, 84 kDa |
| Observed molecular weight | 84 kDa |
| GenBank accession number | BC148802 |
| Gene Symbol | RPS6KA6 |
| Gene ID (NCBI) | 27330 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK32 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for RSK4 antibody 21877-1-AP | Download protocol |
| WB protocol for RSK4 antibody 21877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |